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. 2025 Jan 2;15:310. doi: 10.1038/s41598-024-81393-z

Fig. 3.

Fig. 3

NMN increased NADP(H) levels and decreased ROS levels in oocytes. (A) NADP(H) levels increased in oocytes cultured with 100 μM NMN. A total of 2,449 oocytes was used to analyze NADP(H) values in three independent runs. P < 0.01 by t-test. (B) Oocytes cultured with 0–100 μM NMN were stained with 1 μM H2DCFDA and the amount of hydrogen peroxide (H2O2) in the oocytes was compared. A tendency for ROS levels in oocytes to decrease with increasing NMN concentrations in the culture medium, especially at 10 μM NMN or higher, was visible. The experiment was repeated four times, sampling 9–10 oocytes per trial. In total,198 oocytes were used for the analysis in four independent runs. a-cP < 0.05 by ANOVA followed by the Tukey–Kramer test. Numbers in parentheses indicate the number of oocytes used in the measurements. The scale bar is 25 μm.