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. 2025 Jan 2;16:4. doi: 10.1038/s41467-024-55445-x

Fig. 2. The spectral monitoring of the aggregation of ESi5 dyes with different water-soluble groups.

Fig. 2

a The chemical structures of ESi5-NS, ESi5-N, ESi5-S, and ESi5-C1(-C5). b The electrostatic potential surfaces calculated for ESi5-NS, ESi5-N, ESi5-S and ESi5-C1 at B3LYP/6-31G level. c The UV-Vis absorption (Blue, solid line) and the fluorescence emission (Red, dash line) spectra of the resulting solution upon dilution of the MeOH stock of ESi5-NS, ESi5-N, ESi5-S, and ESi5-C1 in PBS (pH = 7.4, 10 mM). The UV-Vis absorption (Blue, space-filled) and emission spectra (Red, space-filled) of ESi5 in MeOH were plotted for comparison purposes.