Fig. 1. Identification of KIF18A as a vulnerability of CIN^High tumors.

DEPMAP²³ gene essentiality data was used to identify genes required for proliferation of CIN^High cells. a Categorization of CIN across 1660 cell lines as CIN^High (top quartile), CIN^Med or CIN^Low (bottom quartile) as measured by fraction genome altered (FGA). Data presented as mean values. Each dot represents 1 cell line. b Volcano plot visualizing the odds ratio from a Fisher’s Exact test to determine the association between RNAi essentiality scores and CIN, which identified genes that are significantly essential in CIN^High cell lines (FDR < 0.25). Each dot represents one gene. Genes in green are more essential in CIN^High cells, genes in red are more essential in CIN^Low cells, and genes in blue did not show differential essentiality. KIF18A p value = 0.0072 with a Fisher’s Exact test. c Cell lines requiring KIF18A for proliferation (Ess) exhibit higher median FGA compared to cell lines not requiring KIF18A for proliferation (Non-Ess) based on RNAi essentiality scores across 748 cell lines. Data presented as median (center) with 25th to 75th percentile bounds (boxes), minimum and maximum (whiskers). **p = 0.006 by unpaired, 2-tailed t test. Source data are provided in the Source Data file.