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. 2025 Jan 2;16:307. doi: 10.1038/s41467-024-55300-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — a Growth inhibition as measured by CellTiter-Glo of indicated cancer and non-transformed epithelial cell lines after treatment with VLS-1272 for 168 h. Data presented as individual values from n = 2 biological replicates. b Proliferation of MDA-MB-231 cells over-expressing KIF2A (CIN^High) or KIF2B (CIN^Low) treated with VLS-1272 and assessed by CellTiter-Glo after 168 h of treatment. Data presented as individual values from n = 2 biological replicates. c Growth inhibition of primary T cells isolated from healthy donors after stimulation with αCD3/CD28 beads, treated with VLS-1272 and assessed by CellTiter-Glo after 72 h. Data presented as individual values from n = 2 donors. Dead cell (propidium iodide-positive cells) enumeration by live cell imaging in the presence of vehicle or indicated VLS-1272 concentration for 150 h in JIMT-1 cells (d) or CAL51 cells (e). Data presented as mean -/+ SD from n = 3 biological replicates. f Dead cell quantification by acridine orange/propidium iodide staining following 3-day treatment with the indicated concentrations of VLS-1272 in OVCAR-3 cells. Data presented as mean -/+ SD from n = 3 biological replicates. P values are calculated compared to untreated control and labeled on the corresponding bar, using one-way ANOVA with Dunnett’s multiple comparisons test. g Western blot of cleaved Caspase 3 (MW: 17 kDa) versus full length Caspase 3 (MW: 35 kDa) after 24- and 72 h treatment of CAL51 and OVCAR-3 cells with VLS-1272. One representative experiment of 2 biological replicates is shown. Growth inhibition as measured by CellTiter-Glo of HCC15 (h) and HCC1806 cells (i) after treatment with the indicated concentration of VLS-1272 with or without 0.5 or 2 nM BAY-1217389 (MPS1 inhibitor) for 3 days. Data presented as mean values -/+ SD from n = 4 biological replicates. Source data are provided in the Source Data file.