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Osteogenic differentiation of BMSCs cultured on the gradient coating. (a) Optical images of ALP activity of BMSCs after osteogenic induction for 3 and 7 days. (b) Optical images of collagen type I secreted by BMSCs after osteogenic induction for 7 and 14 days. (c) Optical images of mineralization nodule of BMSCs after osteogenic induction for 14 and 21 days.
