Table 4. Performance of the purification process of AA involving treatment of DM-DPM medium with the MB20 resin in batch (scenario 2) and a step-elution with H2SO4.
Parameter | DPM | DM-DPM | DM-DPM MB20 (2–7)c | DM-DPM MB20 (2–6)d |
---|---|---|---|---|
Yield (%) stepa | 100 | 91.0 | 90.3 | 75.3 |
Yield (%) aggr.b | 100 | 91.0 | 82.2 | 68.5 |
Purity (%) | 2.4 | 59.8 | 96.9 | 99.2 |
Purity increase | 1.0 | 24.6 | 40.4 | 41.3 |
Enrichment factor | 1.0 | 58 | 1256 | 5086 |
Yield in a step with respect to the previous one.
Aggregate yield in a step with respect to the starting DPM medium.
MB20 (2–7), pooled fractions from elution steps 2 to 7.
MB20 (2–6), pooled fractions from elution steps 2 to 6.