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. 2025 Jan 3;25:9. doi: 10.1186/s12870-024-06027-y

Fig. 3.

Fig. 3

RT-PCR amplification of Tombusviridae family using universal primer of Tombusviridae family (CarmoIIF/CarmVIR) (Lanes 1–5) and specific primer for PFBV (CH2/CH1) (Lanes 6–10). M: GenRuler™ 1 kb DNA ladder (Fermentas); Lanes 1–3: Pelargonium samples; Lane 4: Negative control (plantlet derived from meristem tip culture of Pelargonium), Lane 5: water, 6–8: PFBV specific primer on Pelargonium samples; Lane 9: negative control, Lane 10: water