Table 2.
The sequences of the primers and Taqman probes used in the present study.
| Sequence | Exon Location | RefSeqNumber | ||
|---|---|---|---|---|
| human RPLP0 | Probe | 5’-/56-FAM/CCCTGTCTT/ZEN/CCCTGGGCATCAC/3IABkFQ/−3’ | 2–3 | NM_001002 |
| Primer2 | 5’-TCGTCTTTAAACCCTGCGTG-3’ | |||
| Primer1 | 5’-TGTCTGCTCCCACAATGAAAC-3’ | |||
| human COL1A1 | Probe | 5’-/56-FAM/TCGAGGGCC/ZEN/AAGACGAAGACATC/3IABkFQ/−3’ | 1–2 | NM_000088 |
| Primer2 | 5’-GACATGTTCAGCTTTGTGGAC-3’ | |||
| Primer1 | 5’-TTCTGTACGCAGGTGATTGG-3’ | |||
| human COL4A1 | Probe | 5’-/56-FAM/TCATACAGA/ZEN/CTTGGCAGCGGCT/3IABkFQ/−3’ | 51–52 | NM_001845 |
| Primer2 | 5’-AGAGAGGAGCGAGATGTTCA-3’ | |||
| Primer1 | 5’-TGAGTCAGGCTTCATTATGTTCT-3’ | |||
| human COL6A1 | Probe | /56-FAM/CAGGTTTCG/ZEN/GTCACAGCGGTAGT/3IABkFQ/ | 2–3 | NM_001848 |
| Primer2 | 5’-CCTCGTGGACAAAGTCAAGT-3’ | |||
| Primer1 | 5’-GTGAGGCCTTGGATGATCTC-3’ | |||
| human FN1 | Probe | /56-FAM/TACAGCTTA/ZEN/TTCTCCCTCGCCCAG/3IABkFQ/ | 3–4 | NM_212482 |
| Primer2 | 5’-CGTCCTAAAGACTCCATGATCTG-3’ | |||
| Primer1 | 5’-ACCAATCTTGTAGGACTGACC-3’ | |||
| human ACTA2 | Probe | 5’-/56-FAM/AGACCCTGT/ZEN/TCCAGCCATCCTTC/3IABkFQ/−3’ | 8–9 | NM_001613 |
| Primer2 | 5’-AGAGTTACGAGTTGCCTGATG-3’ | |||
| Primer1 | 5’-CTGTTGTAGGTGGTTTCATGGA-3’ | |||
| human IL6 | Probe | 5’-/56-FAM/CAACCACAA/ZEN/ATGCCAGCCTGCT/3IABkFQ/−3’ | 4–5 | NM_000600 |
| Primer2 | 5’-GCAGATGAGTACAAAAGTCCTGA-3’ | |||
| Primer1 | 5’-TTCTGTGCCTGCAGCTTC-3’ | |||
| human IL1b | Probe | 5’-/56-FAM/AGAAGTACC/ZEN/TGAGCTCGCCAGTGA/3IABkFQ/−3’ | 1–3 | NM_000576 |
| Primer2 | 5’-CAGCCAATCTTCATTGCTCAAG-3’ | |||
| Primer1 | 5’-GAACAAGTCATCCTCATTGCC-3’ | |||
| human CCL2 | Probe | 5’-/56-FAM/AGACGCGGA/ZEN/CGATGATGTGAACAC/3IABkFQ/−3’ | 6–7 | NM_004360 |
| Primer2 | 5’-AGCAGCCACCTTCATTCC-3’ | |||
| Primer1 | 5’-GCCTCTGCACTGAGATCTTC-3’ | |||
| human LOX | Probe | 5’-/56-FAM/CACAATTTC/ZEN/ACCGTATTAGAAGGCAAAGCA/3IABkFQ/−3’ | 6–7 | NM_002317 |
| Primer2 | 5’-TTCCCACTTCAGAACACCAG-3’ | |||
| Primer1 | 5’-ACATTCGCTACACAGGACATC-3’ | |||
| human CTGF | Probe | 5’-/56-FAM/CAGCCAGAA/ZEN/AGCTCAAACTTGATAGGC/3IABkFQ/−3’ | 4–5 | NM_001901 |
| Primer2 | 5’-GCTCGGTATGTCTTCATGCTG-3’ | |||
| Primer1 | 5’-GAAGCTGACCTGGAAGAGAAC-3’ | |||
| human MMP2 | Probe | 5’-/56-FAM/TTCTGTCCC/ZEN/CATGAAGCCCTGTTC/3IABkFQ/−3’ | 6–7 | NM_004530 |
| Primer2 | 5’-TCCACCACCTACAACTTTGAG-3’ | |||
| Primer1 | 5’-GTGCAGCTGTCATAGGATGT-3’ | |||
| human MMP3 | Probe | 5’-/56-FAM/AGCTTCAGT/ZEN/GTTGGCTGAGTGAAAGA/3IABkFQ/−3’ | 4–5 | NM_002422 |
| Primer2 | 5’-TGAACAATGGACAAAGGATACAAC-3’ | |||
| Primer1 | 5’-TGAGTGAGTGATAGAGTGGGT-3’ | |||
| human MMP9 | Probe | 5’-/56-FAM/CCAGGAGGA/ZEN/AAGGCGTGTGC/3IABkFQ/−3’ | 3–4 | NM_004994 |
| Primer2 | 5’-ACATCGTCATCCAGTTTGGTG-3’ | |||
| Primer1 | 5’-CGTCGAAATGGGCGTCT-3’ | |||
| human MMP14 | Probe | 5’-/56-FAM/TTGTTCCTC/ZEN/AAAGTGCCTGTTTGCTC/3IABkFQ/−3’ | 1–1 | NM_004995 |
| Primer2 | 5’-TTCGCCGACTAAGCAGAAG-3’ | |||
| Primer1 | 5’-CTTGAATTCCTAGACCGCTGT-3’ |