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. 2024 Dec 11;111:105479. doi: 10.1016/j.ebiom.2024.105479

Fig. 3.

Fig. 3

Non-adjuvanted and poly-ICLC-adjuvanted CD40.CoV2 vaccine elicits polyepitopic CD8+T cells with a different pattern of antigen-dominance. (a) Percentages of cytokines-secreting CD8+ T cells for the total reactivity in response to SARS-CoV-2 OLPs in spleen cells of HIS-mice after background subtraction. Data were analyzed using the Mann–Whitney test. Median and individual values from two independent experiments are shown. ∗∗p < 0.01. (b) Proportion of cytokines-secreting CD8+ T cells specific to vRBD, vS1, vS2 and vN induced by the non-adjuvanted or poly-ICLC-adjuvanted CD40.CoV2 vaccine. (c) Correlation between SARS-CoV-2 specific cytokines+ CD4+ T cells (%) and cytokines-secreting CD8+ T cells (%) for the total reactivity. Statistical comparisons were performed using Spearman's correlation. (d) Examples of FACS plots of HLA-A∗0201-RBD multimer staining performed on HIS-mouse spleens, gated on human CD3+ T cells. The negative control for the RBD multimer staining was obtained by gating on mouse CD45+ cells. (e) Percentages of HLA-A∗0201-RBD multimer+ human CD8+ T cells in HIS-mouse spleens. Data were analyzed using the Mann–Whitney test. Median and individual values from two independent experiments are shown. ∗p < 0.05.