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. 2024 Dec 10;14:101860. doi: 10.1016/j.toxrep.2024.101860

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© 2024 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — (a, b, c) shows the immunohistochemical staining for Caspase-3 in heart ventricle tissue. 4a: There aren't many positive cytoplasmic immune reactions in Group I (Control) or Group III (Selenium and Vitamin E Treatment), which means there aren't many cell apoptosis events. 4b: Group II (Azithromycin Treatment) shows a strong positive cytoplasmic immune reaction, highlighting increased apoptosis due to azithromycin exposure. 4c: Group IV (Azithromycin with Selenium and Vitamin E Treatment) has few positive immune reactions, which suggests that giving these drugs together lowers the death of cells. Staining is observed at 400x magnification.