Fig. 5.

(a, b, c) shows the immunohistochemical staining for TNF- in heart ventricle tissue. 5a: There aren't many positive cytoplasmic immune reactions in Group I (Control) or Group III (Selenium and Vitamin E Treatment), which means there isn't much inflammation. 5b: Group II (Azithromycin Treatment) shows strong positive immune reactions, reflecting elevated inflammation associated with azithromycin exposure. 5c: People in Group IV (Azithromycin with Selenium and Vitamin E Treatment) have fewer positive immune responses, which suggests that the selenium and vitamin E are working together to reduce inflammation. Observations were made at 400x magnification.