Fig. 1.

Subcellular localization and expression of the Notch signaling pathway during porcine oocyte meiotic progression. Immunofluorescence analyses of Notch signaling receptors (NOTCH1, NOTCH2, NOTCH3, and NOTCH4) and ligands (JAG1, JAG2, DLL1, DLL3, and DLL4) in porcine cumulus–oocyte complexes (COCs) at (A) 0, (B) 22, (C) 28, and (D) 44 h of in vitro maturation (IVM). Bar = 200 µm. E RT-PCR analyses of Notch signaling receptors (NOTCH1, NOTCH2, NOTCH3, and NOTCH4) and ligands (JAG1, JAG2, DLL1, DLL3, and DLL4) in oocytes and cumulus cells at 0, 22, 28, and 44 h of IVM. Each RT-PCR experiment was performed using oocytes and cumulus cells isolated from 50 COCs