Fig. 5.

Effects of Notch signaling inhibition on the distribution and functions of the mitochondria and endoplasmic reticula in porcine oocytes. A Representative images and (B) fluorescence intensity of MitoTracker-stained oocytes in the indicated groups (n = 78 per group). Bar = 100 µm. C Representative images of mitochondrial distribution in oocytes and (D) the proportions of oocytes with abnormal mitochondrial distribution in the indicated groups (n = 3 per group). Bar = 50 µm. E Representative images and (F) fluorescence intensity (red/green) of JC-1-stained oocytes in the indicated groups (n = 48 per group). Bar = 100 µm. G Representative images and (H) fluorescence intensity of BODYPY-ATP-stained oocytes in the indicated groups (n = 40 per group). Bar = 50 µm. I qRT-PCR-assayed transcript levels of mitochondria-related genes (TFAM, POLG, POLG2, SIRT1, PPARGC1A, CPT1A, and CPT1B) in the indicated groups (n = 3 per group). J Representative images and (K) fluorescence intensity of ER-Tracker-stained oocytes in the indicated groups (n = 118 per group). Bar = 100 µm. L Representative images of endoplasmic reticulum (ER) distribution in oocytes and (M) the proportions of oocytes with abnormal ER distribution in the indicated groups (n = 3 per group). Bar = 50 µm. N Representative images and (O) fluorescence intensity of Fluo-3-stained oocytes in the indicated groups (n = 50 per group). Bar = 100 µm. P Representative images and (Q) fluorescence intensity of Rhod-2-stained oocytes in the indicated groups (n = 80 per group). Bar = 100 µm. R qRT-PCR-assayed transcript levels of ER stress-related genes (HSPA5, ERN1, ATF6, DDIT3, and ATF4) in the indicated groups (n = 3 per group). S Ratios of qRT-PCR-assayed transcript levels of spliced/unspliced XBP1 genes in the indicated groups (n = 3 per group). Data are derived from at least three independent experiments, and different superscripts indicate significant differences (P < 0.05). Con, control; RO, 10 µM RO4929097