Skip to main content
. 2025 Jan 3;11(1):eadr2441. doi: 10.1126/sciadv.adr2441

Fig. 3. OsPUB45 positively regulates rice disease resistance to M. oryzae and Xoo.

Fig. 3.

(A to C) Disease symptoms (A), the relative leaf area with lesions as measured by ImageJ (B), and relative fungal biomass (C) of representative leaves of ospub45 mutants and ZH11 plants after punch inoculation with M. oryzae isolate RB22. (D) Chitin-induced ROS accumulation dynamics in ospub45 mutants and ZH11 plants. Water treatment (Mock) was the negative control. (E and F) Relative transcript levels of defense-related genes OsPAL1 (E) and OsPR10 (F) in ospub45 mutants and ZH11 plants at 0 and 24 hours after spray inoculation with M. oryzae isolate RB22 as determined by qRT-PCR. (G to I) Disease symptoms (G), the relative leaf area with lesions as measured by ImageJ (H), and relative fungal biomass (I) of representative leaves of OsPUB45-OX and ZH11 plants after punch inoculation with M. oryzae isolate RB22. (J) Chitin-induced ROS accumulation dynamics in OsPUB45-OX and ZH11 plants. Water treatment (Mock) was the negative control. (K and L) Relative transcript levels of defense-related genes OsPAL1 (K) and OsPR10 (L) in OsPUB45-OX and ZH11 plants at 0 and 24 hours after spray inoculation with M. oryzae isolate RB22 as determined by qRT-PCR. (M and N) Phenotypes of ospub45 mutants inoculated with the Xoo isolate PXO99A (M) and lesion length (N). Scale bar, 2 cm. (O and P) Phenotypes of OsPUB45-OX plants inoculated with the Xoo isolate PXO99A (O), and lesion length (P). Scale bar, 2 cm. (Q) Time-course degradation of OsCSN5-GFP in rice protoplasts from ZH11 or ospub45 mutants. Cotransfected rice protoplasts were treated with 100 μM CHX to block protein synthesis for the indicated duration of time, and OsCSN5-GFP levels were monitored by immunoblotting. All the statistical analysis data are shown as mean ± SE, and significance was determined at *P < 0.05 and **P < 0.01 (n = 3) with a two-tailed Student’s t test.