Fig. 3.

HGF production by NSCLC cells and inhibition of lymphocyte activation in the presence of pembrolizumab (“Pembro”) A expression levels of HGF in NSCLC cell cultures were assessed using an ELISA assay. B Peripheral blood mononuclear cells (PBMCs) were stained with CFSE and activated with phytohemagglutinin (PHA) alone or in combination with pembrolizumab. C PHA-activated cells were cultured either alone or on a primary NSCLC monolayer in the presence of pembrolizumab and/or crizotinib. After 96 h of incubation, CFSE-stained cells were collected and further stained with PE-Cy7-conjugated anti-CD3, BV421-conjugated anti-CD45, and APC-conjugated anti-CD8 antibodies. The cells were analyzed using flow cytometry (B). The presented data are representative of at least 6 patients