Fig. 3.

O-GlcNAc transferase (OGT) regulates the MOF levels. (A) Immunofluorescence staining of MOF (red) and Ki-67 (green) in MOVAS cells or siMOF-transfected MOVAS or siOGT-transfected MOVAS cells with/without Thiamet G (Thia). (B) Quantification of MOF mean fluorescence. CON, n=63; CON+Thia, n=82; siOGT, n=44; siOGT+Thia, n=42; siMOF, n=40; and siMOF+Thia (n=31). Data are presented as the mean±SD. **P<0.01, ***P<0.001, ****P<0.0001. ns, non-significant. n indicates the cell numbers. CON, control; MOF, males absent on the first; MOVAS, mouse vascular smooth muscle cells.