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. 2024 Dec 10;30:101401. doi: 10.1016/j.mtbio.2024.101401

Fig. 6.

Fig. 6

(a) Transmitted light photography images of MDA-MB-468 cells measured in AFM contact mode. Black arrows indicate analyzed cell. (b) Total cellular stiffness of MDA-MB-468 cells treated with EGF for 24 and 48 h. (c) Nuclear stiffness of MDA-MB-468 cells treated with EGF for 24 and 48 h (c). Total cellular stiffness of HCC1954 cells treated with TGF-β1 for 48 h. (d) Nuclear stiffness of MDA-MB-468 cells treated with TGF-β1 for 48 h. (e) Anisotropy of actin fibers of control HCC1954 cells compared with TGF-β1 stimulated cells (t-test; ∗∗P < 0.01). Confocal images of (f) untreated and (g) TGF-β1-treated HCC1954 cells showing nuclei (blue) and actin cytoskeleton (red) at 63× magnification. Right panel (zoom-in) highlights actin cytoskeleton architecture to either show a cross-linked network (f) or parallel stress fibers (g). Stars indicate statistical significance (∗∗∗P < 0.001; ns = not significant).