Fig. 5.

Effect of YBX1 in MC3T3-E1 cells on ferroptosis through the ATF4/FSP1 axis. (A) YBX1, ATF4, and FSP1 protein expression levels in MC3T3-E1 cells transfected with shYBX1 or ATF4 overexpression vectors, shYBX1 plus ATF4 overexpression vectors, or shNC plus NC. (B) YBX1, ATF4, and FSP1 protein expression levels and quantification in high-glucose (HG)-treated MC3T3-E1 cells transfected with shYBX1 or ATF4 overexpression vectors, shYBX1 plus ATF4 overexpression vectors, or shNC plus NC. (C) Cellular Fe²⁺ concentrations in HG-treated MC3T3-E1 cells transfected with shYBX1 or ATF4 overexpression vectors, shYBX1 plus ATF4 overexpression vectors, or shNC plus NC. (D) Lipid ROS levels in HG-treated MC3T3-E1 cells transfected with shYBX1 or ATF4 overexpression vectors, shYBX1 plus ATF4 overexpression vectors, or shNC plus NC. n = 3. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, and *** P < 0.001. YBX1, Y-box binding protein 1; ATF4, activating transcription factor 4; FSP1, Ferroptosis suppressor protein 1; ROS, reactive oxygen species