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Results of 144 h fed-batch fermentation with 2 L working volume. Lactose (40 g/L) and glucose (20 g/L) were used for initial media. Glucose was maintained at 20 g/L, and a pH-stat mode was employed for its continuous supplementation. The 2’-FL overproducing strain, BMZF11, was used for 2’-FL synthesis. Black circle, cell growth; Black square, 2’-FL (g/L); Black triangle, D-lactose (g/L)
