Fig. 3.

Electromyography of homozygous ANXA11-P36R knock-in mice. A Representative images of denervative potentials detected in mutant mice, including fibrillation potentials in the left shoulder-deltoid muscle (SDM), positive sharp waves (PSWs) in the left erector spinae muscle (ESM) and right gastrocnemius muscle (GAS), and spontaneous and giant motor unit potentials in the right GAS and right rectus femoris muscle (RFM). B Representative CMAP responses in the forelimbs and hindlimbs of wild-type (+ / +) and mutant (R/R) mice. Left, representative CMAP images in wild-type and mutant mice at different ages. Right, peak-to-peak CMAP amplitudes in wild-type and mutant mice. Both mutant and wild-type mice exhibited motor development from 4 to 10 months. CMAP amplitudes decreased significantly in mutant mice over time, particularly in hindlimbs from 10 to 12 months, whereas amplitudes remained stable in wild-type mice (Two-way ANOVA with Sidak’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; n = 3 mice per group)