Fig. 7.

Progressive autophagy impairment in homozygous ANXA11-P36R knock-in mice. Autophagy markers, including Beclin-1, LC3BII/I, and p62, were analyzed in the cortex of 2-, 4-, and 9-month-old mutant mice, revealing a progressive decline in autophagic activity. Representative immunoblots demonstrate the impairment of autophagy in mutant mice, with quantified levels normalized to GAPDH. Statistical analysis was conducted using one-way ANOVA, with data presented as mean ± SEM (n = 4 biologically independent replicates per group). Significance levels: *p < 0.05, **p < 0.01, ***p < 0.001