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. 2025 Jan 4;15:743. doi: 10.1038/s41598-024-84356-6

Table 2.

Tentatively identified hydrocarbons (H1 to H25) in the cuticle and tergal glands of Z. cucumis. Note that the percentages of hydrocarbons in this table are calculated using the sum of both volatile compounds and hydrocarbons. MM: molar mass; RI: Kovats retention index calculated by using n-alkane series; RI (ref): RI from the literature; MC: male cuticle; FC: female cuticle; MTG: male tergal gland; FTG: female tergal gland.

No Hydrocarbons MM RI RI (Ref) Diagnostic ions MC/% FC/% MTG/% FTG/% Ref
H1 10-MeC28 408.8 2830 280/282, 154/155 0.28 0.15 0.20 0.24
H2 2-MeC28 408.8 2860 2860 364/365 1.45 1.52 3.28 4.81
H3 6,10-DiMeC28 422.8 2870 322/323, 98/99, 252/253, 168/169 0.31 0.23 0.25 0.33
H4 2,10-dimethylC28 422.8 2894 406/407, 280/281, 168/169 6.57 6.90 7.53 10.18
H5 2,6-dimethylC28 422.8 2901 406/407, 336/337, 112/113 2.51 4.03 3.48 5.44
H6 11-MeC29 422.8 2927 2931 280/281, 168/169 12.22 12.48 11.28 15.03 21
H7 7-MeC29 422.8 2940 2945 336/337, 112/113 2.45 2.84 2.39 4.01 21
H8 5-MeC29 422.8 2948 2952 364/365, 84/85 0.73 0.85 2.71 2.89 21
H9 9,13-DiMeC29 436.9 2958 2963 322/323, 140/141, 252/253, 210/211 1.91 1.87 0.24 0.52 58
H10 7,11-DiMeC29 436.9 2964 2965 350/351, 112/113, 280/281, 182/183 2.87 2.84 1.11 0.76 21
H11 3-MeC29 422.8 2974 2974 392/393, 56/57 0.13 0.18 1.96 3.34 58
H12 5,11-DiMeC29 436.9 2980 2983 378/379, 84/85, 280/281, 182/183 1.56 1.63 0.79 1.75 58
H13 3,11-DiMeC29 436.9 3004 406/407, 56/57, 280/281, 182/183 2.43 2.82 0.73 1.65
H14 6-MeC30 436.9 3041 3044 364/365, 98/99 2.06 2.49 4.07 8.83 21
H15 4-MeC30 436.9 3060 3058 392/393, 70/71 0.23 0.37 10.26 19.98 21
H16 2,12-; 2,14-DiMeC30 450.9 3094 435, 280/281, 196/197; 435, 252/253, 224/224 3.26 5.41 2.28 4.21
H17 2,6-DiMeC30 450.9 3201 406/407, 70/71, 364/365, 112/113 8.38 12.83 3.77 9.01
H18 13-MeC31; 15-MeC31 450.9 3127 3130 280/281, 196/197; 224/225, 252/253 3.01 4.71 < 0.01 < 0.01 21
H19 7-MeC31 450.9 3137 3140 364/365, 112/113 4.67 6.25 < 0.01 < 0.01 21
H20 9,13-DiMeC31 464.9 3156 3162 352/353, 140/141, 280/281, 210/211 1.12 1.06 0.34 0.88 58
H21 7,13-DiMeC31 464.9 3163 378/379, 112/113, 280/281, 210/211 0.30 0.25 0.39 1.36
H22 3- Me-C31 450.9 3174 3174 420/421 0.32 0.31 0.03 < 0.01 21
H23 3,13-DiMeC31 464.9 3202 434/435, 280/281, 210/211 0.60 0.82 0.02 < 0.01
H24 3,7-DiMeC31 464.9 3204 434/435, 364/365, 126/127 0.57 1.09 < 0.01 < 0.01
H25 4,12-DiMeC32 478.9 3290 70/71, 308/309, 196/197; 0.55 0.44 < 0.01 < 0.01