Table 1.

Primers were Used to Determine the Expression of hsa-miR-16-1

mi-RNA	(5′ >3′) sequence	(C º)
U6 internal control R primer	5-CTCGCTTCGGCAGCACA-3	59/1
USTL primer	5-GAAGGCGAGGAGCAGATCGAGGAAGAAGACGGAA	90
	GAATGTGCGTCTCGCCTTCTTTCNNNNNNNN-3
hsa-miR-16-1-3p	5-CCAGUAUUAACUGUGCUGCUGA-3
hsa-miR-16-1-3P-F primer	5-ACACTCCAGCTGGGCCAGTATTAACTGTGCTGCTG-3	60
hsa-miR-16-1-5p	5-UAGCAGCACGUAAAUAUUGGCG-3
hsa-miR-16-1-5P-F primer	3-5-ACACTCCAGCTGGGTAGCAGCACGTAAATATTGGC	60
Universal Revers primer	5-TGGTGTCGTGGAGTCG-3	59/8
U6 internal control F primer	5-AACGCTTCACGAATTTGCGT-3	59/1