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. 2025 Jan 4;16:20417314241311073. doi: 10.1177/20417314241311073

Figure 4.

Figure 4.

SPI1 regulated the stemness and chondrogenic potential of BMSCs. (a) Western blot assessed the protein expression of SPI1 on rat BMSCs after lentivirus infection. n = 3. (b) CCK-8 was performed to explore the proliferative capacity of YBMSCs, YBMSCs after knockdown of SPI1, OBMSCs, and OBMSCs after overexpression of SPI1. n = 3. (c) The colony-forming abilities of YBMSCs, YBMSCs after knockdown of SPI1, OBMSCs, and OBMSCs after overexpression of SPI1 were explored by crystal violet after culture for 7 days. n = 3. (d-e) After osteogenic induction for 21 days, ARS and ALP staining was performed in YBMSCs, YBMSCs after knockdown of SPI1, OBMSCs, and OBMSCs after overexpression of SPI1. n = 3. (f) After chondrogenic induction for 21 days, alcian blue staining was performed in YBMSCs, YBMSCs after knockdown of SPI1, OBMSCs, and OBMSCs after overexpression of SPI1. n = 3. (g and h) Representative immunofluorescence images and quantitative analysis of SOX9 (green) and COL2A1 (red) in cartilage regeneration organoids. DAPI (blue) staining of nuclei. n = 3. (i and j) Representative qRT-PCR and WB of SOX9, COL2A1, ACAN,SPI1 on four distinct types of cartilage regeneration organoids. n = 3. *p < 0.05. **p < 0.01. ***p < 0.001, ns: no significance. Values are means ± SD.