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. 2025 Jan 4;16:20417314241311073. doi: 10.1177/20417314241311073

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© The Author(s) 2025

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — In vivo implantation of SPI1-overexpressed cartilage regeneration organoids could enhance cartilage repair in the old rats. (a) Representative images of H&E, Safranin O/Fast green staining of sections at 4 weeks after organoids implantation. n = 6. (b and c) Macroscopic scores and histological scores for assessment of the cartilage regeneration at 4 weeks after organoids implantation. n = 6. (d) Representative images of IHC staining of sections at 4 weeks after organoids implantation. n = 6. (e and f) Quantification of COL2A1 and COL1A2 positive cells ratio in articular regenerated cartilage area. n = 6. (g) Representative images of sirius red staining of sections at 4 weeks after organoids implantation. n = 6. (h) Quantification of type I and III collagen ratio in articular regenerated cartilage area. n = 6. *p < 0.05. **p < 0.01. ***p < 0.001, ns: no significance. Values are means ± SD.