Fig. 3.

Schematic representation of insertion sequences (ISs) observed within the mgrB gene. Four ISs were identified: ISKpn26, IS903B, ISKpn74 and ISKpn14. The Red lines on mgrB represent the insertion sites. The directions of the insertion events are shown as colorful arrows, with the IS lengths marked above each IS. Underlined nucleotide sequences were direct repeats (DRs). Triangles flanking each IS were left and right inverted repeats (IRLs and IRRs). (1) For the six isolates harboring the ISKpn26 insertion: five had insertions at position + 74 (A); one had an insertion at -64 bp, located in the promoter region upstream of the start codon (B); (2) among the three isolates with the IS903B insertion: two displayed insertions at + 94 (C); one revealed an insertion at + 120 (D); (3) for the three isolates bearing the ISKpn74 insertion: two demonstrated insertions at + 69 bp (E); one presented an insertion at + 94 bp (F); (4) among the three isolates with the ISKpn14 insertion: one showed an insertion at + 51 bp (G); two had insertions at -52 bp, situated in the promoter region upstream of the start codon (H)