Table 1. Summary of the frequencies obtained with different bioinformatic workflows.

Bioinformatic workflow	Total frequencies	Number of featuresa	Minimum frequency by sample	Maximum frequency by sample	Mean frequency by sample	Median frequency by sample	Mean Good’s coverage (%)
Geneious+ UNITE	34,739	3,138	395	2,059	965	848	79.83
Single MiCoP	93,589	210	1,194	5,043	2,600	2,392	99.32
Paired MiCoP	1,281,436	234	21,654	52,713	35,595	35,008	99.94
Kraken2	1,021,469	87	17,680	42,316	28,374	27,185	100

Note:

^aTaxonomic species (single and paired MiCoP, and Kraken2) or operational taxonomic unit at 97% of similarity (Geneious+UNITE).