Table 2. p-values of environmental variables resulted from ‘envfit’ using a canonical correlation analysis (CCA) on the Hellinger-transformed sequence data.

Environmental variable	Geneious+UNITE	Single MiCoP	Paired MiCoP	Kraken2
OMa	0.015 b	0.004	0.029	0.001
Ca	0.001	0.001	0.492	0.001
Silt	0.001	0.001	0.060	0.033
WHCc	0.001	0.001	0.105	0.378
Fe	0.064	0.481	0.118	0.451
K	0.038	0.080	0.786	0.786
Mg	0.001	0.001	0.015	0.084
pH	0.001	0.001	0.001	0.001
Cu	0.056	0.322	0.256	0.383
Clay	0.296	0.335	0.621	0.312
P	0.021	0.114	0.051	0.577
N	0.240	0.001	0.012	0.152

Notes:

^aOM, Organic material.

^bValues in bold are significant (p < 0.05) and were retained for environmental distances analysis.

^cWHC, water holding capacity.