Table 1. Primer sequences and conditions used in quantitative polymerase chain reaction (PCR).
| Target organism | Standard strain | Primer sequence (5′-3′) | PCR product siza (bp) | 3-step amplification | Reference | ||
|---|---|---|---|---|---|---|---|
| Denaturation | Annealing | Elongation | |||||
| Prevotella spp. | Faecalibacterium prausnitzii | F: CACRGTAAACGATGGATGCC | 527–529 | 94℃ for 20 s | 55℃ for 20 s | 72℃ for 30 s | [28] |
| JCM31915 | R: GGTCGGGTTGCAGACC | ||||||
| Lactobacillus spp. | Lactobacillus plantarum | F: AGCAGTAGGGAATCTTCCA | 341 | 95℃ for 15 s | 58℃ for 20 s | 72℃ for 30 s | [29] |
| 22A-3 | R: CACCGCTACACATGGAG | ||||||
| Bifidobacterium spp. | Bifidobacterium | F: TCGCGTC(C/T)GGTGTGAAAG | 549–563 | 95℃ for 15 s | 58℃ for 20 s | 72℃ for 30 s | [29] |
| longumJCM1217 | R: CCACATCCAGC(A/G)TCCAC | ||||||
| Fusobacterium spp | Fusobacterium nucleatum | F: CCCTTCAGTGCCGCAGT | 248 | 95℃ for 15 s | 61℃ for 15 s | 72℃ for 30 s | [29] |
| JCM8532 | R: GTCGCAGGATGTCAAGAC | ||||||
| Bacteroides spp. | Bacteroides thetaiotaomicron | F: GGTGTCGGCTTAAGTGCCAT | 495 | 95℃ for 15 s | 68℃ for 20 s | 72℃ for 30 s | [29] |
| DSMZ 2079 | R: CGGATGTAAGGGCCGTGC | ||||||
| Faecalibacterium | Faecalibacterium prausnitzii | F: GGAGGAAGAAGGTCTTCGG | 248 | 95℃ for 15 s | 60℃ for 15 s | 72℃ for 30 s | [30] |
| prausnitzii | DSMZ 17677 | R: AATTCCGCCTACCTCTGCACT | |||||