Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid	Description	Reference
Strain
L. paracasei IGM394	Wild-type, derivative of L. paracasei IGM393	[17]
L. paracasei BKN191	sirA deleted, derived from IGM394	[16]
E. coli JM109	Cloning host for pGEM®-T Vector and pLP401t	Takara
E. coli MC1061	Cloning host for pG+host5, hsdR, mcrB, araD139, Δ(araABC-leu)7679, ΔlacX74, galU, galK, rpsL, thi	Lucigen Co.
E. coli EC101	Cloning host for pG+host5, Kmr, E. coli JM101 with repA from pWV01 integrated in chromosome	[25]
Plasmid
pG+host5	Emr, thermosensitive E. coli-Lactic acid bacteria shuttle vector	[26]
pgRNA-bacteria	Plasmid for guide RNA transcription	[3]
pGCasRsirA	pG+host5 containing Spcas9 and sgRNA targeting sirA	This study
pGCasRslpA	pG+host5 containing Spcas9 and sgRNA targeting slpA	This study
pGdCas9	pG+host5 containing Spcas9 with D10A and H840A mutations	This study
pGEM®-T Vector	Ampr, Cloning vector	Promega
pGEM::sirA	pGEM®-T Vector containing sirA	This study
pLP401-T	Ampr, Emr, Expression vector containing the pAmy promoter	[27]
pLPCasRsirA	pLP401-T containing Spcas9 and sgRNA targeting sirA	This study
pBTE	Ampr, Emr, thermosensitive E. coli- Lactic acid bacteria shuttle vector	[28]
pBTE::dcas9	pBTE containing L. paracasei Cas locus with Ppgm promoter and dcas9	This study
pGRsirA-up	pG+host5 containing sgRNA targeting sirA (up)	This study
pGRsirA-down	pG+host5 containing sgRNA targeting sirA (down)	This study