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. 2024 Oct 28;27(12):111258. doi: 10.1016/j.isci.2024.111258
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-IgG(Fc), Human, Goat-Polyclonal Antibody Bethyl Laboratories Cat# A80-304A;
RRID: AB_10634115
Anti-IgG(Fc), Human, Goat-Poly, HRP Bethyl Laboratories Cat# A80-304P; RRID: AB_10631811
Anti-IgM(Fc), Human, Goat-Poly Bethyl Laboratories Cat# A80-100A;
RRID: AB_67079
Anti-IgM(Fc), Human, Goat-Poly, HRP Bethyl Laboratories Cat# A80-100P;
RRID: AB_67082
Anti-Igλ Light Chain, Human, Goat-Poly Bethyl Laboratories Cat# A80-116A;
RRID: AB_67093
Anti-Igλ, Human, Goat-Poly, HRP Bethyl Laboratories Cat# A80-116P;
RRID: AB_67591
Anti-IgA(α), Human, Goat-Poly Bethyl Laboratories Cat# A80-102A;
RRID: AB_67044
Anti-IgA, Human, Goat-Poly, HRP Bethyl Laboratories Cat# A80-102P;
RRID: AB_67047
Anti-IgE(ε), Human, Goat-Poly Bethyl Laboratories Cat# A80-108A;
RRID: AB_67053
Anti-IgE, Human, Goat-Poly, HRP Bethyl Laboratories Cat# A80-108P;
RRID: AB_67056
PE anti-mouse/human CD45R/B220 Antibody Biolegend Cat# 103207;
RRID: AB_312992
PE/Cyanine7 anti-mouse/human CD45R/B220 Biolegend Cat# 103221;
RRID: AB_313004
BD Horizon™ BUV661 Rat Anti-Mouse CD19 BD Biosciences Cat# 612971;
RRID: AB_2870243
BD Horizon™ PE-CF594 Mouse Anti-Human IgM BD Biosciences Cat# 562539;
RRID: AB_2737641
PE/Cyanine7 anti-human IgG Fc Antibody Biolegend Cat# 410721;
RRID: AB_2750226
PE/Cyanine7 anti-human Ig light chain λ Antibody Biolegend Cat# 316622; RRID: AB_2687262
Pacific Blue™ anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody Biolegend Cat# 144613; RRID: AB_2563291
BD OptiBuild™ BV650 Rat Anti-Mouse CD38 BD Biosciences Cat# 740489;
RRID: AB_2740212
PE/Cyanine7 anti-mouse CD138 (Syndecan-1) Antibody Biolegend Cat# 142513;
RRID: AB_2562197
PE anti-mouse CD267 (TACI) Antibody Biolegend Cat# 133403;
RRID: AB_2203542
Bacterial and virus strains
XL10-Gold Ultracompetent Cells Agilent Cat# 200315
Chemicals, peptides, and recombinant proteins
Antibiotic G-418 Sulfate Promega Cat#V7983
Blasticidin S FUJIFILM Wako Pure Chemical Corporation Cat# 029-18701
Hygromycin B FUJIFILM Wako Pure Chemical Corporation Cat# 089-06151
HAT Media Supplement (50×) Hybri-Max™ Sigma-Aldrich Cat# H0262-10VL
Lipofectamine™ 2000 Transfection Reagent ThermoFisher Scientific Cat# 11668027
Sigma Adjuvant System Sigma-Aldrich Cat# S6322
Freund's Complete Adjuvant BD Cat# DF0638-60-7
Ni-NTA Agarose QIAGEN Cat# 30210
Critical commercial assays
Human IgG Subclass ELISA Kit ThermoFisher Scientific Cat# 99-1000
Expi293™ Expression System Kit ThermoFisher Scientific Cat# A14635
Deposited data
Repertoire analysis of mice producing fully human antibody with lambda light chain This paper DDBJ: PRJDB18270
Experimental models: Cell lines
Chinese hamster ovary (CHO) (Hprt-/-) cells JCRB Cell Bank JCRB0218;
RRID:CVCL_8545
CHO-K1 RIKEN BRC RCB0285;
RRID:CVCL_0214
DT40 RIKEN BRC RCB1464;
RRID:CVCL_0249
Endogenous Ig knockout (HKLD) mouse embryonic stem cells This paper N/A
Experimental models: Organisms/strains
Mouse:ICR:Jcl:ICR CLEA RRID: IMSR_JCL:JCL:mOT-0001
Mouse:C57BL/6N CLEA RRID:MGI:3055581
Mouse:HKLD:STOCK Ighmtm1YakazIgkctm1YakazIglc1rs32383745-A Satofuka et al.14 N/A
Mouse:IGHL:STOCK Tc(MAC;IGL;IGH)1Yakaz IghmIghmtm1YakazIgkctm1YakazIglc1rs32383745-A This paper N/A
Oligonucleotides
hChr.22: arm for loxP insertion HindIII553La L
5'-TGTAGCTGACTTTAGCCACCCACAAGTAC-3'
AscI553La R 5'-TCGAGGCGCGCCCTCAAACTC
CTGGGTGTAAATGATCCTCCTGC-3' (5,743 bp)		 This paper N/A
hChr.22: arm for loxP insertion KpnI553Ra L 5'-TGAG
GGTACCGTGCAGTAAAGTATGATTGAGC-3'
SalI553Ra R 5'-TCGAGTCGACCTTGCTGATTA
TACCTCATCTCCTTCCCTC-3' (3,880 bp)		 This paper N/A
hChr.22: arm for FRT insertion BamHISL350La L
5'-TCGAGGATCCGGCCTCCCAAAGGATTAT
AGACGTGAGCCACTGTAscISL350La R-3'
AscISL350La R 5'-TCGAGGCGCGCCGGC
ACCTCTCCTATTTTCTTCACAGCACTT-3'
(3,595 bp)		 This paper N/A
hChr.22: arm for FRT insertion AscISL350Ra L 5'-TCGAG
GCGCGCCAGCATGGTGGCCCGCACGTATAGTCGC
AGCTA-3' NotISL350Ra R 5'-TCGAGCGGCCGCAAA
GAAGGGGCCCGCCTCTGCCTCTAAATCC
TGAC-3' (3,872 bp)		 This paper N/A
hChr.22: loxP targeting check 22CeT La L 5'-CCTG
CCTTCTTGTTTCAGCTCTCAACTG-3' 22CeT La
R 5'-GACGTGCTACTTCCATTTGTCACGTCCT-3'
(4,794 bp)		 This paper N/A
hChr.22: loxP targeting check 22CeT Ra L
5'-ATCCCCATGTGTATCACTGGCAAAC
TGT-3' 22CeT Ra R 5'-ACACTTTAGTCC
CTGTCCCCTCAACGAG-3' (4,791 bp)		 This paper N/A
hChr.22: FRT targeting check 22TeT Ra L
5'-AGCAGAGCTCGTTTAGTGAACCGT
CAGA-3' 22TeT Ra R 5'-CTGTCCTATC
CTTGCAGCTGTCTTCCAG-3' (4,911 bp)		 This paper N/A
hChr.22 region check SERPIND1 L 5'-ACCT
AGAGGGTCTCACCTCC-3' SERPIND1 R
5'-CCCTGGACATCAAGAATG-3' (223 bp)		 This paper N/A
hChr.22 region check 553P-F 5'-AGATCTC
TTGAGCCCAGCAGTTTGA-3' 553P-R
5'-TGAAGTTAGCCGGGGATACAG
ACG-3' (454 bp)		 This paper N/A
hChr.22 region check PPM1F L 5'-AACGG
CAGCCAAACCAAAGA-3' PPM1F R 5'-ACC
AGGACTGGCTGGGCATA-3' (207 bp)		 This paper N/A
hChr.22 region check IGLVI-70 L 5'-AGTC
TGCGCTGACCCAGGAA-3' IGLVI-70 R
5'-TTGAGCCAGAGAAGCGGTCA-3' (195 bp)		 This paper N/A
hChr.22 region check hVpreB1-F 5'-TGTCCTG
GGCTCCTGTCCTGCTCAT-3' hVpreB1-Rm
5'-GGCGGCGACTCCACCCTCTT-3' (589 bp)		 This paper N/A
hChr.22 region check IgL-F 5'-GGAGACCAC
CAAACCCTCCAAA-3' IgL-Rm 5'-GAGAGTTG
GAGAAGGGGTGACT-3' (482 bp)		 This paper N/A
hChr.22 region check GNAZ L 5'-TCCACTTGG
GGGTCTGCATT-3' GNAZ R 5'-TGGTGCTGA
GCAGCTGTGTG-3' (202 bp)		 This paper N/A
hChr.22 region check 344-F 5'-ATCATCTGCT
CGCTCTCTCC-3' 344-R 5'-CACATCTGTAGT
GGCTGTGG-3' (450 bp)		 This paper N/A
hChr.22 region check hλ5-F 5'-AGCCCCAAG
AACCCAGCCGATGTGA-3' hλ5-R 5'-GGCAG
AGGGAGTGTGGGGTGTTGTG-3' (529 bp)		 This paper N/A
hChr.22 region check hVpreB3-F 5'-CACTGC
CTGCCCGCTGCTGGTA-3' hVpreB3-R 5'-GG
GCGGGGAAGTGGGGGAGAG-3' (511 bp)		 This paper N/A
hChr.22 region check 350P-F 5'-ACCAGCGC
GTCATCATCAAG-3' 350P-R 5'-ATCGCCAG
CCTCACCATTTC-3' (461 bp)		 This paper N/A
hChr.22 region check LIF L 5'-TGGGACTTA
GGTGGGCCAGA-3' LIF R 5'-GCCTCCCCA
AGAGCCTGAAT-3' (199 bp)		 This paper N/A
IGL expression Vλ3LEA1 5'-CCCCCAAGCTT
GCCTGGACCCCTCTCTGG-3'		 This paper N/A
IGL expression Cλ1 5'-GGGAATTCGGGTAG
AAGTCACTGATCAG-3'		 This paper N/A
IGL expression Vλ3JLEAD 5'-ATCGGCAAAG
CTTGGACCCCTCTCTGGCTCAC-3'		 This paper N/A
IGL expression Vλ3BACK4 5'-CCCCCAAGCT
TCTCGGCGTCCTTGCTTAC-3'		 This paper N/A
IGL expression Cλ7 5'-GGGAATTCGGG
TAGAAGTCACTTACGAG-3'		 This paper N/A
cDNA synthesis BSL-18E 5'-AAAGCGGC
CGCATGCTTTTTTTTTTTT TTTTTTVN-3'		 Kitaura et al.34 N/A
Adaptor P10EA 5'-GGGAATTCGG-3' Kitaura et al.34 N/A
Adaptor P20EA 5'-TAATACGACT
CCGAATTCCC-3'		 Kitaura et al.34 N/A
IgG constant region CG1 5'-CACCT
TGGTGTTGCTGGGCTT-3'		 Kitaura et al.34 N/A
Second PCR CB2 5'-AGGCAGTATCT
GGAGTCATTGAG-3'		 Kitaura et al.34 N/A
Second PCR CG2 5'-TCCTGAGGACT
GTAGGACAGC-3'		 Kitaura et al.34 N/A
Second PCR P22EA-ST1 5'-GTCTCGTGG
GCTCGGAGATGTGTATAAGAGACAGC
TAATACGACTCCGAATTCCC -3'		 Kitaura et al.34 N/A
Second PCR CB-ST1-R 5'-TCGTCGGCAG
CGTCAGATGTGTATAAGAGACAGGCT
CAAACACAGCGACCTC-3'		 Kitaura et al.34 N/A
Second PCR P22EA-ST1 5'-GTCTCGTGGG
CTCGGAGATGTGTATAAGAGACAGCTAA
TACGACTCCGAATTCCC -3'		 Kitaura et al.34 N/A
Second PCR CG-ST1-R 5'-TCGTCGGCAGCG
TCAGATGTGTATAAGAGACAGTGAGTTCC
ACGACACCGTCAC-3'		 Kitaura et al.34 N/A
Recombinant DNA
IGH-BAC BACPAC resource center CH17-212P11
IGL-BAC BACPAC resource center CH17-95F2
RBD expression vector This paper N/A
5′ HPRT-loxP-PGKHyg unit Satofuka et al.14 N/A
5′ HPRT-FRT-CMVBsd unit Satofuka et al.14 N/A
pBS185 CMV-Cre Addgene RRID:Addgene_11916
pCAG-FLPo vector Satofuka et al.14 N/A
Software and algorithms
Metafer4 slide scanning platform software V3.10.5 Meta Systems https://metasystems-international.com/en/products/metafer/
Cytexpert version 2.3 Beckman Coulter https://www.beckman.jp/flow-cytometry/software
Isis Fluorescence Imaging System software V5.4.12 Meta Systems https://metasystems-international.com/
R software version 3.4.2 The R Project for Statistical Computing https://www.r-project.org/
IGBLAST NIH https://www.ncbi.nlm.nih.gov/igblast/
IMGT IMGT https://www.imgt.org/
CLASTALW GenomeNet https://www.genome.jp/tools-bin/clustalw
Principal component analysis was performed using the prcomp-function in R (version 3.4.2) RDocumentation https://www.rdocumentation.org/packages/stats/versions/3.6.2/topics/prcomp
R software using circos open-source software circos https://circos.ca/
Biotek Gen5 version 3.03 Agilent https://www.chem-agilent.com/contents.php?id=1006877
Graphpad Prism 10 Graphpad https://www.graphpad.com/updates/prism-1000-release-notes
Kaluza software version2.1. Beckman Coulter https://www.beckman.jp/flow-cytometry/software/kaluza