Table 2.
The advantages, disadvantages and applications of separation methods for EV subpopulations.
| Separation Methods for EV Subpopulations | Advantages | Disadvantages | Applications | |
|---|---|---|---|---|
| Microfluidic Chip-Based Method | Rapid, automatic, compatible with multiple indicators | Low throughput, unsuitable for large-scale sample | Small volume, liquid biopsy | |
| Click Chemistry/Covalent Chemistry-Based Method | Reversible, relatively high integrity and biological activity | Complex procedure, biological properties slightly altered | Downstream research | |
| Aptamer-Based Method | Multivalent DNA Nanoflowers | High efficiency, high affinity, traceless release | Complex procedure, limited accuracy | Downstream research, liquid biopsy |
| Robust Separation of Double-Positive EVs | Multiple indicators, traceless release | Low concentration | Fine liquid biopsy and downstream research | |
| Receptor-Ligand Interaction-Based Method | Novel, simple | Low specificity and limited generality | Specific protein separation | |
| Negative Separation Strategy-Based Method | Novel, theoretically undamaged, high biological activity | Relatively low purity | Downstream research and liquid biopsy | |