Figure 1.

Optofluidic device for the NAD(P)H imaging of live mouse embryos. (a) Schematic of the optofluidic device showing the coupling of the micropipette tips in the inlet and the outlet of the microchannel. The NAD(P)H imaging is through light-sheet fluorescence microscopy. The light-sheet is formed on-chip with a micro-lens and an optical fibre (scanning area), and the fluorescence signal is recorded with an off-chip objective lens. (b) Microscopic photograph of the device illustrating three two-cell mouse embryos travelling from the micropipette tip inlet to the microchannel. (c) Microscopic photograph of the device showing two-cell mouse embryos passing the light-sheet. Scale bars 50 µm. NAD(P)H, nicotinamide adenine dinucleotide; PDMS, polydimethylsiloxane.