Fig. 6.

KLF4 mediated the transcriptional regulation of RNF112. A The transcription factors predicted by TFtarget (http://bioinfo.life.hust.edu.cn/hTFtarget#!/) to bind to the RNF112 promoter region were intersected with 766 common DEGs. Venn diagram showed the common DEGs. B Real-time PCR was used to detect the expression of KLF4 in CRC and adjacent tissues. C The correlation between the expression of KLF4 mRNA and RNF112 mRNA in mRNA sequencing and CRC clinical samples was analyzed. D The expression of KLF4 and RNF112 in SW620 cells was determined by real-time PCR and western blot. E Luciferase activity in SW620 cells was tested. Potential binding sites of KLF4 on the RNF112 promoter region were also shown. F Binding of KLF4 on the RNF112 promoter region in SW620 cells was determined by Ch-IP. (n = 3). p < 0.01. p < 0.001. p < 0.0001