Table 1.
Key phenotypic properties of cellulotrophic bacteria and their saccharolytic satellites obtained from cellulose enrichments from Siberian soda lakes.
| Property | Aerobic | Anaerobic | ||||||
|---|---|---|---|---|---|---|---|---|
| ABcell2 | ABcell3 | ANBcel5 | ANBcel31 | ANBcel28 | ANBcel2 | ANBcel1 | ANBcel3 | |
| Taxonomy* | Cellvibrionales | Cytophagales | Chitivibrionales | Clostridiales | Halanaerobiales | Marinilabiales | Balneolales | |
| Cell morphology | ||||||||
| Shape | Curved motile rods | Flexible rods | Motile vibrio | Plump rods | Flexible rods | Flexible rods | Motile rods | Motile curved rods |
| Size (μm) | 1-4 x 0.4-0.5 | 3-12 x 0.2-0.4 | 1.5-5 x 0.3-0.4 | 2-6 x 0.7-1.0 | 2-12 x 0.1-0.15 | 2-6 x 0.15 | 1.5-2.0 x 0.5 | 1.5-3.0 x 0.5 |
| Cyst like cells | − | + | + | − | − | − | − | − |
| Endospores | − | − | − | + | + | − | − | − |
| Pigmentation | Yellowish | - | - | Yellow-orange | Yellow | Pink | Yellowish | Yellowish |
| Growth with: | ||||||||
| Cellulose | − | + | + | + | + | − | − | − |
| Barley β-glucan | + | - | w+ | − | + | − | − | − |
| Xyloglucan | + | w+ | − | + | − | − | + | w+ |
| Lichenan | + | − | + | − | + | − | + | − |
| Laminarin | + | − | − | + | − | + | + | − |
| Glucomannan | + | − | − | − | − | + | + | + |
| Beta-mannan | − | − | − | − | w+ | − | − | − |
| Xylan | + | − | − | + | + | + | + | w+ |
| Cellobiose | + | w+ | + | + | nd | + | + | + |
| Starch | + | − | − | − | − | + | + | w+ |
| Microaerobic growth** | + | + | − | − | − | − | + | − |
| H2 formation on cellulose/cb | nd | nd | − | + | + | Trace | − | − |
Cellulotrophs are in bold, *Based on complete 16S rRNA gene sequences; ** statically at 2% O2 in the gas phase; nd – not detected.