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. 2024 Dec 23;14:1504440. doi: 10.3389/fonc.2024.1504440

Table 3.

Summary of non-high-grade glioma pathologies reported in surgical case series of pediatric brainstem biopsy.

Study Details of included patients and reasons for conducting biopsy (if provided) Histological subtypes identified (emphasis on non-glioma findings)
Roujeau 2007 (42) 24 children all with ‘diffuse pontine lesions’ 22 children had WHO III/IV gliomas. 1 child had pilocytic astrocytoma, and 1 had WHO II astrocytoma. In these low-grade lesions, up-front radiation was not used and these children were not enrolled in a trial investigating a chemotherapeutic agent for malignant tumors.
Pirotte 2007 (43) 20 children with ‘intrinsic brainstem tumors’. Biopsies were being conducted because of atypical features (either lateralization within the brainstem or strong contrast enhancement) 2 patients had pilocytic astrocytomas (1 lesion was subependymal in the pons, the other was lateralized within the pons), 2 lateralized pontine lesions were called ‘PNET’s
Schumacher 2007 (5) 142 cases of pediatric ‘brainstem disease’, analysis conducted to find correlation between radiographic diagnosis from MRI (3 radiologists) and final histopathological diagnosis The radiologists correctly identified non-tumor disease 50-75% of the time. They correctly identified gliomas 85-91% of the time.
Patel 2009 (44) 24 stereotactic brainstem biopsies 8 patients had pilocytic astrocytomas, 2 had tuberculous lesions, 1 had an epidermoid cyst, and 1 had infarction. No details of radiographic features of these lesions were provided.
Haegelen 2010 (45) 6 biopsies in children with brainstem lesions 1 patient had gliosis (imaging characteristics not provided)
Dellaretti 2011 (46) 44 children with intrinsic brainstem lesions Of the patients with diffuse lesions (29), non-glioma pathologies were found in 2 patients: 1 ependymoma and 1 ganglioglioma
Ogiwara 2013 (47) 7 biopsies for ‘intrinsic brainstem lesions’ in children, reason for biopsy was “atypical” feature (extension beyond the boundary of the pons, or well-margined localized enhancing portion). 1 patient had PNET, 1 patient had pilocytic astrocytoma
Manoj 2014 (48) 41 children underwent stereotactic biopsy for brainstem lesions 3 cases of pilocytic astrocytoma, 4 tuberculomas, 1 “other inflammatory”. Authors note “all the biopsies in diffuse non-enhancing lesions were gliomas”
Quick-Weller 2017 (49) 5 children with intrinsic brainstem lesions suspected to be high grade gliomas 1 child had astrogliosis, T-cell infiltration and mineralization without tumor cells, this child was observed clinically with no progression. A second child was found to have pilocytic astrocytoma. A ‘wait and see’ strategy was adopted and the lesion showed remission on radiographic follow-up.
Dawes 2018 (50) 11 robotic brainstem biopsies in children 1 patient had an inflammatory lesion (imaging characteristics not provided)
Gupta 2020 (51) 22 robotic brainstem biopsies in children 2 had pilocytic astrocytomas (imaging characteristics not provided)
Peruzzi 2024 (52) Case report of a 19 year old female with MRI consistent with DIPG Biopsy showed lesional cells were negative for H3K27M alterations, sequencing revealed frameshift mutation in NF1 thought to be likely driver mutation