Fig. 2. Experimental setup. A) Sample holder with a red-light emitting diode used for sample visualization during the writing procedure. B) Microstructures' array fabricated on the glass coverslip, visible after the development procedure. C) UV-light spot, used to cure a biocompatible glue, and visible during the sealing procedure of the glass coverslip to the chamber's lid. D) Microstructures' array included in the bioreactor's chamber inside a lid. E) CAD model of one microstructure composing the array, measuring 500 × 500 × 40 μm3. Design of the elementary unit (red arrow) constituted by two pores, each measuring 50 × 50 × 20 μm3. F) SEM image of one microstructure. G) Confocal laser scanning microscopy image of the entire microstructures' array. H) MOAB assembled, positioned on an optical microscope (Olympus IX70, Japan) and illuminated by a fluorescent mercury lamp filtered by a Semrock BrightLine TRITC-A fluorescent filter (Olympus, Japan). I) Scheme of the assembled hydraulic circuit: a high precision syringe pump was used to independently perfuse the three chambers of the bioreactor. At the outlet three reservoirs collected the waste products.
