Fig. 6. Immunofluorescence and collagen I fluorescence intensity quantification of static and dynamic co-cultures at day 7. A) Confocal images of the stained static co-cultures. Flat substrate on the left, microstructures on the right. RFP, collagen I, and CD31 are visible in red, green and cyan, respectively. B) Confocal images of the stained dynamic co-culture. Flat area on the side of the microstructures' array on the left, microstructures on the right. RFP, collagen I and CD31 are visible in red, green and cyan, respectively. C) Quantification of collagen I synthesized by RFP-HDF under treated and untreated conditions, on flat substrates in static experiments. Data are normalized with respect to the control (0.1% BSA), n ≥ 27 for each condition. D) Quantification of collagen I synthesized by RFP-HDF at day 7 in the flat area surrounding the microstructures in dynamic experiments, in the presence of VEGF or TGF-β1. Data are normalized respect to the control (0.1% BSA), n ≥ 27 for each condition. ****p-value < 0.0001. E) Quantification of collagen I synthesized by RFP-HDF at day 7 on 3D substrates in static experiments, in the presence of VEGF or TGF-β1. Data are normalized respect to the control (0.1% BSA), n ≥ 27 for each condition. ***p-value < 0.001; ****p-value < 0.0001. F) Quantification of collagen I synthesized by RFP-HDF at day 7 on 3D substrates in dynamic experiments, in the presence of VEGF or TGF-β1. Data are normalized respect to the control (0.1% BSA), n ≥ 27 for each condition. ****p-value < 0.0001. The green box in the graphical sketches (not scaled) above images indicates regions of the samples where acquisitions were performed.