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. 2025 Jan 1;81(Pt 1):1–10. doi: 10.1107/S2053230X24011348

Table 2. Crystallization.

Method Vapor diffusion, sitting drop
Plate type Tray 101-d6, 96-well plates
Temperature (K) 290
Protein concentration (mg ml−1) 14.88
Buffer composition of protein solution 20 mM HEPES pH 7.0, 300 mM NaCl, 5%(v/v) glycerol, 1 mM TCEP, 0.5 mM IMP-1088, 0.5 mM Myr-CoA
Composition of reservoir solution 27% PEG 3350, 200 mM ammonium sulfate, 100 mM bis-Tris pH 6.0
Volume and ratio of drop 0.4 µl, 1:1
Volume of reservoir (µl) 80