| Method |
Vapor diffusion, sitting drop |
| Plate type |
Tray 101-d6, 96-well plates |
| Temperature (K) |
290 |
| Protein concentration (mg ml−1) |
23.6 |
| Ligand mixture composition |
6 mM MgCl2, 6 mM ATP, 6 mM biotin |
| Buffer composition of protein solution |
20 mM HEPES pH 7.0, 300 mM NaCl, 5%(v/v) glycerol, 1 mM TCEP |
| Composition of reservoir solution |
0.1 M sodium citrate tribasic–citric acid pH 4.0, 0.8 M ammonium sulfate (JCSG+ condition B1) |
| Volume (µl) |
0.4 |
| Ratio of drop |
1:1 |
| Volume of reservoir (µl) |
80 |
| Composition of cryoprotectant solution |
0.075 M sodium citrate tribasic–citric acid pH 4.0, 0.6 M ammonium sulfate, 25%(v/v) ethylene glycol |
