FIGURE 4.

Clonogenic assay showed the long‐term effects of combination treatment of CRC cells with PRIMA‐1^met (PRI) and L‐OHP. (A) HCT116 (upper panel) and DLD‐1 (lower panel) cells were seeded in soft agar in 12‐well plates and. Culture medium containing DMSO, PRIMA‐1^met, L‐OHP or the combination at indicated doses were replaced twice per week for 2 weeks. A photograph of each well in a representative experiment was shown. (B) Cell colonies comprising more than 8 cells were counted under a low‐magnification microscope (Nikon Corporation, Japan). Data were shown from three independent experiments, and the error bars represented the SEM. *p < 0.05; **p < 0.01.