Figure 3.

Macrophage autophagy deficiency enhances renal inflammation in mice with AKI. (A and B) immunofluorescence and quantification of ADGRE1⁺ macrophages in renal tissues of mice with SA-AKI. (n = 6-10 per group). (C and D) Representative western blot images and quantification of pro-inflammatory CCL2, NLRP3, and IL18 in the renal tissues of mice treated with LPS (1 mg/kg) for 24 h. (n = 4-6 per group). (E and F) immunofluorescence and quantification of ADGRE1⁺ macrophages in renal tissues of mice subjected to IR for 3 days. (n = 5-7 per group). (G and H) Representative western blot images and quantification of pro-inflammatory CCL2, NLRP3, and IL18 in the renal tissues of mice with ir-induced AKI. (n = 4-7 per group). (I and J) Representative western blot images and quantification of pro-inflammatory cytokines, NOS2, NLRP3, and IL18, in BMDMs subjected to LPS (100 ng/mL) and IFNG (50 ng/mL) for 24 h. (n = 3 per group). All data are presented as mean ± SEM. Two-way ANOVA followed by Tukey’s post hoc test (B, D, F, H); unpaired Student’s t-test (J). Scale bars: 50 μm.