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. 2024 Dec 11;16(1):2440554. doi: 10.1080/21505594.2024.2440554

Table 3.

Significantly downregulated histone encoding genes following treatment of THP-1 macrophages with A. veronii AS1 or E. coli K-12.

Gene Symbol Gene Name A. veronii AS1 log2FC (p-adj) E. coli K-12 log2FC (p-adj)
H1–3 H1.3 linker histone, cluster member −1.12 (3.58E–28) −0.70 (3.27E–13)
H2AC13 H2A clustered histone 13 −1.03 (4.64E–10) −0.67 (2.00E–04)
H2AC16 H2A clustered histone 16 −1.15 (2.44E–08) −0.80 (6.17E–05)
H2AC17 H2A clustered histone 17 −1.03 (7.85E–17) −0.56 (3.55E–06)
H2AC4 H2A clustered histone 4 −1.43 (6.95E–14) −0.43 (1.93E–01)
H2AC7 H2A clustered histone 7 −1.07 (9.34E–08) −0.67 (8.84E–04)
H2BC10a H2B clustered histone 10 −1.63 (1.01E–28) −1.37 (9.29E–19)
H2BC13 H2B clustered histone 13 −1.01 (5.83E–10) −0.64 (4.72E–05)
H2BC14 H2B clustered histone 14 −1.11 (2.60E–12) −0.72 (3.04E–08)
H2BC17 H2B clustered histone 17 −1.12 (3.76E–23) −0.54 (1.95E–06)
H2BC6 H2B clustered histone 6 −1.03 (8.10E–18) −0.81 (3.65E–10)
H2BC9 H2B clustered histone 9 −1.15 (2.68E–17) −0.97 (8.53E–12)
H3C1 H3 clustered histone 1 −1.09 (4.24E–09) −0.97 (4.86E–07)
H3C11 H3 clustered histone 11 −1.04 (8.48E–07) −0.70 (1.28E–04)
H3C12 H3 clustered histone 12 −1.42 (1.02E–16) −0.99 (1.85E–13)
H3C2 H3 clustered histone 2 −1.03 (5.71E–18) −0.73 (2.32E–12)
H3C3 H3 clustered histone 3 −1.21 (5.10E–35) −0.91 (1.63E–25)
H3C4 H3 clustered histone 4 −1.03 (2.72E–12) −0.79 (3.32E–10)
H3C7 H3 clustered histone 7 −1.03 (1.44E–4) −0.34 (4.75E–01)
H3C8a H3 clustered histone 8 −1.70 (1.49E–18) −1.15 (2.32E–15)
H4C2 H4 clustered histone 2 −1.04 (9.50E–08) −0.35 (1.70E–01)
H4C9 H4 clustered histone 9 −1.60 (2.36E–19) −0.87 (2.73E–06)

aGenes significantly downregulated by both A. veronii AS1 or E. coli K-12 treated THP-1 macrophages.

Twenty-two biologically significantly downregulated histone encoding genes (p-adj <0.05, log2FC ≤ −1) were identified through RNA-seq analysis of THP-1 macrophages treated with A. veronii AS1 or E. coli K-12 for 2 hours. Two of these genes (H3C8 and H2BC10) are significantly downregulated by both bacteria, with 20 only significantly downregulated in A. veronii AS1 treated cells. Gene symbols and names were recorded. The log2FC and p-adj for both bacterial species are recorded and are listed adjacent. Statistical significance was assessed using Wald’s test and then adjusted using the Benjamini–Hochberg method.