Figure 4. Cell-intrinsic IFN-γ induced signaling in oligodendroglial lineage promotes EAE pathogenesis.

(A) Flow cytometry analysis indicates Ifngr1 deletion in OPCs cultured from Olig2-Cre Ifngr1^fl/fl mice as indicated. OPCs were cultured from embryonic pups of Ifngr1^fl/fl mice and Olig2-Cre Ifngr1^fl/fl genotypes.

(B) Mean clinical score of EAE in Ifngr1^fl/fl mice and Olig2-Cre Ifngr1^fl/fl mice induced by active immunization with MOG_35–55 (p < 0.05, two-way ANOVA).

(C) Disease stage-specific activities in EAE influenced by specific Ifngr1 ablation in oligodendroglial lineage cells.

(D) Hematoxylin and eosin (H&E) staining, anti-CD3 immunostaining, and myelin basic protein (MBP) staining were performed as indicated on lumbar spinal cords harvested 25 days after immunization.

Data are from one experiment representative of three independent experiments, with n = 5/group in each experiment. Error bars indicate SEM; *p < 0.05, **p < 0.01.