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. 2024 Dec 21;29(1):24–34. doi: 10.1080/19768354.2024.2442401

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — The conditioned media of macrophages primed with Pm facilitates the progression of CRC cells. (A) CCK8 assay was performed to examine the proliferation of CRC cells in response to CM (conditioned media) and CM-Pm (conditioned media with Pm infection) from THP-1 macrophages compared to normal media for 24, 48, and 72 h. (B) Cisplatin resistance was examined in CRC cells after 48 h treatment with CM, CM-Pm, or control. (C-F) Wound healing assays were conducted, and wound closures (%) were assessed to measure the mobility of CRC cells following 24, 48 h treatment with CM, CM-Pm, or control. Scale bar: 100 µm. (G and H) Transwell assay was conducted to examine the migration ability of CRC cells following treatment with CM, CM-Pm, or control. All data are displayed as the mean ± SD. **P < 0.01, ***P < 0.001, and ****P < 0.0001.