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The production of the pro-inflammatory cytokine IL-8 significantly increases in macrophages treated with Pm. (A) qRT-PCR was conducted to measure the mRNA levels of representative EMT and migration-related cytokines. (B) ELISA was used to analyze the concentration of IL-8 in the supernatant of THP-1 macrophages treated with Pm at an MOI of 200 for 24 and 48 h. All data are displayed as the mean ± SD. *P < 0.05 and **P < 0.01.
