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IL-8 neutralization reverses the effect of CM-Pm on inducing migration in CRC cells. HT-29 and HCT-116 were co-cultured with CM, CM-Pm, or CM-Pm with IL-8 Ab (500 ng/mL). (A-D) Wound healing assays were conducted to assess the mobility of CRC cells. (E and F) Transwell assay was performed to evaluate the migration of CRC cells. Scale bar: 100 µm. All data are displayed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
