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. 2002 Jul 1;30(13):2809–2816. doi: 10.1093/nar/gkf384

Figure 4.

Figure 4

Kinetics of DnaB helicase activity and modulation by SSBs. The standard helicase assay was carried out over time periods ranging from 0 to 30 min utilizing the 5′→3′ forked substrate. The reaction mixtures contained 27 nM DnaB protein and SSBs as indicated. Lanes 1–5, DnaB protein in the absence of SSB proteins. Lanes 6–10, DnaB protein in the presence of 0.69 µM E.coli SSB. Lanes 11–15, DnaB protein in the presence of 21 nM yRPA. Lanes 16–20, DnaB protein in the presence of 75 nM gp32 protein.