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Effects of SSBs on the kinetics of DnaB ATPase activity. Standard ATPase assays were carried out over time periods ranging from 0 to 60 min, using 3.33 nM DnaB protein in the presence of 200 pmol ssDNA and 0.35 µM SSB, 0.75 µM gp32 and 0.21 nM RPA. No SSB (asterisks); yRPA (triangles); gp32 protein (circles); E.coli SSB (squares). Each point represents the average of triplicate determinations.
