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. 2025 Jan 6;15:954. doi: 10.1038/s41598-024-82109-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — SLCO4A1-AS1 expression is upregulated in GBM. (A) The venn diagram shows the intersection of genes up-regulated in MGG4, MGG6 and MGG8 cultured under NBE conditions compared to culturing in serum conditions according to GSE54791 database. (B) Expression of identified genes in GSC and DGC according to GSE54791 database. (C) Expression of identified genes in GBM and normal brain tissue. (D, E) Volcano plot showing DEGs between GSCs and DGCs in 1228 cells (D) and 0308 cells (E) in the GSE4536 database. (F, G) The comparison of SLCO4A1-AS1 expression in GSC 1228 (F) and GSC 0308 (G) cultured under NBE and FBS conditions according to multiple probe sets of GSE4536 dataset. Significant statistical differences between the two groups were assessed using the Mann–Whitney test. NS, not statistically significant; * P < 0.05; ** P < 0.01; *** P < 0.001.